July 4th, 2007
•Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
Related Videos
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Protocol for Mosquito Rearing (A. gambiae)
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
MISSION esiRNA for RNAi Screening in Mammalian Cells
Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
A Multi-detection Assay for Malaria Transmitting Mosquitoes
RNAi Trigger Delivery into Anopheles gambiae Pupae
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved