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DOI :
10.3791/61908-v
October 28th, 2020
Chapters
0:05
Introduction
0:39
Feces Resuspension and Organic Extraction
1:52
Final RNA Isolation
3:45
Elution of the RNA with Nuclease-Free Water
4:09
Results: Electrophoresis Assay and Nanodrop Analysis of Extracted RNA
5:00
Conclusion
该协议从动物和人类受试者的粪便样本中分离出高质量的总RNA。商业 miRNA 分离试剂盒具有显著适应性,以优化数量和质量分离纯 RNA。RNA分离株适用于大多数下游RNA检测,如测序、微阵列和RT-PCR。
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