Results: Representative Identification of Mosaic G0 Adults and Mutations of Interest
5:07
Conclusion
Transcript
Targeted mutagenesis has been adapted to sandflies only recently and is a crucial technique for understanding the role and function of genes of interest in these vector insects. Embryo microinjection is a very important step in insect genome editi
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This protocol details the steps of CRISPR/Cas9 targeted mutagenesis in sand flies: embryo collection, injection, insect rearing, and identification as well as selection of mutations of interest.