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DOI :
10.3791/61925-v
May 4th, 2021
Chapters
0:05
Introduction
0:41
Differentiation of Murine 3T3-L1 Fibroblasts into Adipocytes and Preparation of Precoated Plates
3:18
Preparation of the Transfection Mix and 3T3-L1 Adipocytes
6:28
Results: Differentiation, Protein Silencing, Micro-RNA Overexpression and Effects of Protein or Micro-RNA Modulation in 3T3-L1 Adipocytes
8:35
Conclusion
여기에 제시된 것은 단백질과 마이크로 RNA 발현을 조절하기 위해 성숙한 아디포사이클로 작은 간섭 RNA(siRNA), 마이크로 RNA 모방(miRs), 또는 항마이크로RNA(anti-miR)와 같은 올리고뉴클레오티드를 전달하는 프로토콜이다.
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