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DOI :
10.3791/61937-v
•
8:59 min
February 24th, 2021
Chapters
0:05
Introduction
0:30
Photopattern DNA onto Aldehyde-Functionalized Slides
2:46
Lift and Label Cells with Cholesterol-Modified DNA
4:21
Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture
7:22
Results: Adhesion of CMO-Labeled Cells to DNA Patterns
8:32
Conclusion
在这里,我们提出了一种使用DNA程序化粘附的单细胞分辨率的微模式细胞方案。该协议使用台式光刻平台在载玻片上创建DNA寡核苷酸的图案,然后用市售的互补寡核苷酸标记细胞膜。寡核苷酸的杂交导致程序化的细胞粘附。
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