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DOI :
10.3791/61954-v
•
8:52 min
December 22nd, 2020
Chapters
0:04
Introduction
0:26
Dissection of Drosophila Larval Brains
2:51
Immobilization on Coverslip with Fibrin Clots
5:58
Addition of Reagents during Live Imaging
7:00
Results: Application of an ATP Analog on Immobilized Egg Chambers During Live Imaging
8:13
Conclusion
このプロトコルは、Fibrin血栓を使用したサンプル固定化のアプリケーション、ドリフトの制限、およびライブイメージング中の試薬の追加と洗い流しを可能にするアプリケーションを記述します。試料は、カバースリップの表面に培養培地を含むフィブリノーゲンの滴に移され、その後、トロンビンを加えることによって重合が誘導される。
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