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DOI :
10.3791/61994-v
November 9th, 2020
Chapters
0:04
Introduction
0:45
Yeast Total Extract Preparation
2:07
Glycerol Gradient Preparation
2:58
Extract Separation and Collection
3:44
Results: Representative Transient Saccharomyces Cerevisiae Spliceosome Subcomplex Interactor Detection
5:15
Conclusion
ここでは、架橋器の存在下でのグリセロール勾配遠心分離であるグラフィックス(勾配固定)の利用について、一過性にスプライセソーム複合体に結合するスプライシング因子間の相互作用を同定する。
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