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DOI :
10.3791/62001-v
•
7:46 min
December 11th, 2020
Chapters
0:04
Introduction
0:53
C. elegans Gonad Preparation and Microinjection
1:56
Injected Worm Recovery and Transfer
2:37
PickingC. elegans for Screening
3:17
Single Worm Lysis and PCR
4:22
Restriction Digestion and Agarose Gel Electrophoresis
5:08
Identification of Positively Edited and Homozygous Edited Worms
6:01
Results: Representative C. elegans rbm-3.2 Gene Editing and Screening
7:10
Conclusion
此协议的目标是使用组装的核糖核蛋白复合物和dpy-10共同 CRISPR 标记进行无缝的 CRISPR/Cas9 编辑,以便进行筛选。 此协议可用于在C. elegans中进行各种基因修改,包括插入、删除、基因替换和 codon 替代。
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