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DOI :
10.3791/62016-v
•
11:42 min
June 10th, 2021
Chapters
0:00
Introduction
1:01
Direct Conversion of Human Fibroblasts to Neuronal Progenitor Cells
4:21
Conversion and iNPC Splitting
6:42
Generating Induced Astrocytes From NPCs
7:43
Astrocyte Differentiation From Frozen iNPC Stocks
8:51
Results: Growth and Characterization of Cells Throughout Conversion Process
10:53
Conclusion
我们描述了一个将人类皮肤衍生的成纤维细胞重新编程为诱导神经元祖先细胞(iNPC)的协议,以及它们随后分化成诱导的星形细胞(iAs)的协议。这种方法可快速、可重复地大量生成 iNC 和 iAs。
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