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DOI :
10.3791/62038-v
April 18th, 2021
Chapters
0:00
Introduction
1:04
Microscope Plate Setup
3:16
Yeast Dilution
6:36
Sonication (Optional)
8:18
Microscope Setup
10:05
Representative Results
11:00
Experimental Pitfalls
12:22
Conclusion
酵母生长表型通过高度平行的延时成像精确测量生长成微殖子的固定细胞。同时,可以监测应力耐受性、蛋白质表达和蛋白质定位,生成综合数据集,研究环境和遗传差异以及同源细胞之间的基因表达异质性如何调节生长。
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