Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/62040-v
•
7:38 min
April 30th, 2021
Chapters
0:05
Introduction
0:44
Preparation and Dissection of Kidney and Calibration of Vibratome Instrument
2:32
Vibratome Sectioning and Kidney Slice Ca2+ Image Acquisition
4:06
Kidney Slice Ca2+ Image Acquisition
5:14
Results: Imaging of Spontaneous Calcium Ion Transient in Vibratome Sections of Whole Kidneys
7:01
Conclusion
このプロトコルの目的は、ビブラートメの切断を使用してマウス腎盂の無傷のペースメーカー領域を分離することです。これらのセクションは、その後、ビブラート細胞内のペースメーカー細胞および他の間質細胞のCa2+一過性特性を解明するために、Situ Ca2+イメージングに使用することができます。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved