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JoVE Journal

Medicine

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Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

Transcript

This protocol explains the necessary steps to perform micro dissection whole-mount immunofluorescence staining and microscopy specifically for the sinus node and AV node in the mouse. The whole-mount methodology is advantageous for addressing the

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We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts.

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