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University Hospital Munich, Ludwig Maximilians University (LMU) Munich

6 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Malek Saleh 1, Mohammed R. Abdullah 1, Christian Schulz 1, Thomas Kohler 1, Thomas Pribyl 1, Inga Jensch 1, Sven Hammerschmidt 1
1Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald

Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

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Medicine

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
Ruibing Xia 1,2,3, Julia Vlcek 1,2, Julia Bauer 1,2,3, Stefan Kääb 1,3, Hellen Ishikawa-Ankerhold 1,2, Dominic Adam van den Heuvel 1,2, Christian Schulz 1,2,3, Steffen Massberg 1,2,3, Sebastian Clauss 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig Maximilians University (LMU) Munich, 2Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig Maximilians University (LMU) Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance

We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts.

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Medicine

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
Ruibing Xia 1,2,3, Simone Loy 1, Stefan Kääb 1,3, Anna Titova 1, Christian Schulz 1,2,3, Steffen Massberg 1,2,3, Sebastian Clauss 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

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Medicine

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice
Philipp Tomsits 1,2,3, Kavi Raj Chataut 1,2, Aparna Sharma Chivukula 1,2, Li Mo 1,2, Ruibing Xia 1,2, Dominik Schüttler 1,2,3, Sebastian Clauss 1,2,3
1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University Munich (LMU)

Here we present a step-by-step protocol for a semiautomated approach to analyze murine long-term electrocardiography (ECG) data for basic ECG parameters and common arrhythmias. Data are obtained by implantable telemetry transmitters in living and awake mice and analyzed using Ponemah and its analysis modules.

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Medicine

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
Manuel Lasch 1,2,3, Mykhailo Vladymyrov 4, Dominic van den Heuvel 1,5, Philipp Götz 1,3, Elisabeth Deindl 1,3, Hellen Ishikawa-Ankerhold 1,5
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital, Ludwig-Maximilians-Universität München, 3Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine, Ludwig-Maximilians-Universität München, 4TKI, University of Bern, 5Department of Internal Medicine I and Cardiology, University Hospital, Ludwig-Maximilians-Universität München

The recruitment of leukocytes and platelets constitutes an essential component necessary for the effective growth of collateral arteries during arteriogenesis. Multiphoton microscopy is an efficient tool for tracking cell dynamics with high spatio-temporal resolution in vivo and less photo-toxicity to study leukocyte recruitment and extravasation during arteriogenesis.

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Medicine

Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice
Philipp Tomsits *1,2,4, Aparna Sharma Chivukula *1,2, Kavi Raj Chataut *1,2, Agus Simahendra 3, Ludwig T. Weckbach 1,2,3, Stefan Brunner 1, Sebastian Clauss 1,2,4
1Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), 2DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), 3Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, 4Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU)

Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice.

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