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DOI :
10.3791/62067-v
December 23rd, 2020
Chapters
0:04
Introduction
0:42
SARS-CoV-2 RNA-FIS in Vero Cells Cultured on Coverslips
2:26
SARS-CoV-2 RNA-FISH in HAE Cultures
3:38
Results: Immuno-RNA-FISH to Detect SARS-CoV-2 RNA in Vero and HAE Cells
4:38
Conclusion
ここでは、その蛍光におけるRNA蛍光をその蛍光(RNA-FISH)と免疫蛍光と組み合わせて、重症急性呼吸器症候群コロナウイルス2(SARS-CoV-2)RNAを可視化する簡単な方法を説明する。このプロトコルは、単一細胞レベルでのSARS-CoV-2 RNA-宿主相互作用の分子特性の理解を深める可能性がある。
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