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DOI :
10.3791/62073-v
December 29th, 2021
Chapters
0:04
Introduction
0:32
Oligonucleotide to Small Nuclear RNA-Activating Protein (SNAP) T7 RNA Polymerase (RNAP) Conjugation and Polyacrylamide Gel (PAGE) Analysis
2:04
Oligonucleotide-Tethered SNAP-T7 Purification
4:13
On-Demand Control Tethered RNAP Activity Demonstration
7:08
Results: Representative DNA-Tethered T7 RNAP Purification and Analysis
8:44
Conclusion
インビトロ転写反応を調節する新規DNAつながされたT7 RNAポリメラーゼの工学について述べています。タンパク質合成と特性評価のステップについて議論し、概念実証の転写制御を検証し、分子コンピューティング、診断、分子情報処理における応用について議論する。
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