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DOI :
10.3791/62088-v
May 22nd, 2021
Chapters
0:04
Introduction
0:50
Preparation of Sucrose Gradient
1:57
Tissue Dissection
3:05
Cell Lysis
4:34
Fractionation and Sample Collection
6:15
Results: Polysome Profiling Analysis of the Developing Mouse Cortex
7:39
Conclusion
哺乳類の脳の発達には、翻訳のレベルでの遺伝子発現の適切な制御が必要です。ここでは、発達中の脳におけるmRNAの翻訳状態を評価するための、組み立て易いショ糖勾配および分画プラットフォームを備えたポリソームプロファイリングシステムについて説明する。
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