In-Nucleus Hi-C in Drosophila Cells

3.9K Views

11:58 min

September 15th, 2021

DOI :

10.3791/62106-v

September 15th, 2021


Transcript

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Hi C

Chapters in this video

0:05

Introduction

1:13

Schneiders Line 2 Plus (S2R+) Drosophila Cell Fixation

2:02

Lysis

3:18

Enzymatic Digestion, DNA End Biotinylation, and Ligation

4:37

Crosslink Reversal and DNA Purification

5:33

Hi-C Template Quality Evaluation

6:26

Biotin Removal/End Repair and Size Selection

7:49

Biotin Pulldown/A-Tailing/Adapter Ligation

9:33

Results: Representative In-Nucleus Hi-C Analysis

11:17

Conclusion

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