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In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

DOI :

10.3791/54516-v

10:31 min

October 15th, 2016

October 15th, 2016

8,567 Views

1Lab of Molecular Genetics, National Heart, Lung and Blood Institute, National Institutes of Health

Drosophila oogenesis continues to be exceptionally useful in the study of mitochondrial proliferation and inheritance. This manuscript describes a detailed protocol used to label the replicating mitochondrial DNA (mtDNA) in Drosophila adult ovaries with 5-ethynyl-2´-deoxyuridine (EdU), which facilitates uncovering mechanisms associated with mitochondrial inheritance that were previously debatable.

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In Situ Labeling

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