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DOI :
10.3791/62146-v
•
9:38 min
February 2nd, 2021
Chapters
0:04
Introduction
0:46
Preparation of Plasmids for the Microinjection Mix
2:33
Microinjection of Embryos from an Anopheles Docking Line
3:38
Crossing and Screening of Transformed Individuals
6:45
Results: Validation of φC31 Single Integration and RMCE in An. stephensi Larvae
8:56
Conclusion
이 프로토콜은 φC31 시스템을 사용하여 Anopheles 말라리아 모기의 게놈에서 사이트 지향적 인 수정을 달성하는 방법을 설명합니다. 설명된 수정은 attP 베어링 도킹 라인의 게놈에 있는 형질전환 카세트의 통합 그리고 교환둘 다 포함됩니다.
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