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DOI :
10.3791/62156-v
•
6:57 min
February 6th, 2021
Chapters
0:04
Introduction
0:51
Preparation of Plates, Chamber Slides and Cell Culture Membrane Inserts
1:30
Crypt Isolation
4:09
Culturing 2D Monolayer
5:17
Results: Reliability of The Primary Epithelial Colon Monolayer Cultures
6:17
Conclusion
在此协议中,我们描述了如何直接从肠道密码生成穆林原发性上皮结肠单层。我们提供实验方法,在透气过滤器上生成汇流单层,在划痕伤口愈合和生化研究中产生汇流单层,以及用于免疫荧光分析的稀疏和共生单层。
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