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DOI :
10.3791/62156-v
•
6:57 min
February 6th, 2021
Chapters
0:04
Introduction
0:51
Preparation of Plates, Chamber Slides and Cell Culture Membrane Inserts
1:30
Crypt Isolation
4:09
Culturing 2D Monolayer
5:17
Results: Reliability of The Primary Epithelial Colon Monolayer Cultures
6:17
Conclusion
本プロトコルでは、腸内窩から直接マウスの一次上皮結腸単層を生成する方法を説明する。透過性フィルター上にコンフルエント単層を生成する実験的アプローチ、傷創傷治癒および生化学的研究のためのコンフルエント単層、免疫蛍光分析のためのまばらでコンフルエントな単層を提供する。
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