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DOI :
10.3791/62165-v
February 9th, 2021
Chapters
0:04
Introduction
0:46
Primary Retinal Ganglion Cell Culture Derived from Zebrafish Embryos
4:45
In Vitro ROS Imaging of Cultured RGC Neurons
6:13
In Vitro ROS Imaging of Developing Embryos
7:40
Results: H2O2 Imaging in Cultured and in Vivo Zebrafish RGCs with roGFP2-Orp1
8:53
Conclusion
このプロトコルは、神経系の発達中にH2O2の生理的シグナル伝達役割を評価するための培養ゼブラフィッシュニューロンおよび幼虫における遺伝的にコードされた過酸化水素(H2O2)バイオセンサーの使用を記述する。それは、異なる細胞タイプに適用し、一般的な開発で活性酸素種(ROS)を研究するために実験的な治療で変更することができます。
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