Name: gold nanoparticle synthesis
Uploaded: 2021-07-10T13:00:00
Description: Watch this Scientific Journal Video about Gold Nanoparticle Synthesis at JoVE.com

作者要感谢弗兰克·奥斯特洛对纳米粒子合成方法的帮助。作者希望感谢国家科学基金会（1807555和203665）和半导体研究公司（2836）的财政支持。

化学量： 注：为了获得纳米粒子合成的适当化学量，请将"纳米粒子合成"表（Osterloh 研究第12条支持信息的第 2 页）上的初始量乘以 3，并稍作修改。 表1 显示了注射液、沸腾溶液、洗涤/净化溶液和金等溶液所需的化学量。
金纳米粒子合成过程的清洁和准备（第1天） 注：以下步骤可在合成过程的第一天完成。
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<ol>
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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+Size+and+Concentration+of+Gold+Nanoparticles+from+UV-Vis+Spectra.">Determination of Size and Concentration of Gold Nanoparticles from UV-Vis Spectra.</a> Analytical Chemistry. 79 (11), 4215-4221 (2007).</li><li>McCold, C. E., Fu, Q., Howe, J. Y., Hihath, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conductance+based+characterization+of+structure+and+hopping+site+density+in+2D+molecule-nanoparticle+arrays.">Conductance based characterization of structure and hopping site density in 2D molecule-nanoparticle arrays.</a> Nanoscale. 7 (36), 14937-14945 (2015).</li><li>Hihath, S., McCold, C., March, K., Hihath, J. 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执行上述的金纳米粒子合成协议应产生直径约 12 nm 且单极性相当高（± 2 nm）的金纳米粒子。然而，有一些关键的步骤和过程参数，可以调整，以潜在地改变金纳米粒子的大小/直径和单分散/多散。例如，将前体溶液注入反应容器并允许四氯酸、油胺和甲苯溶液煮沸两小时后，可以选择立即淬火反应溶液或延迟淬火和自然冷却。如果需要立即淬火，在2小时加热反应步骤完成后，在反应容器中加入10...

金纳米粒子是一种有趣而有用的纳米材料，是许多研究和应用的主题：如生物学1、医学2、纳米技术3和电子设备4。对金纳米粒子的科学研究可追溯到1857年，当时迈克尔·法拉第对金纳米粒子5的合成和特性进行了基础研究。合成金纳米粒子的两种主要"自下而上"技术是柠檬酸盐还原法6、7、8和有机双相合成法9、10。"Turkevich"柠檬酸盐还原法在直径在20纳米以下产生相当单分散的金纳米粒子，但直径在20纳米以上的金纳米粒子的多散率增加：而"布鲁斯特-希夫林"两相法使用硫/硫磺配体稳定生产直径达11纳米的金纳米粒子。使用这些方法预合成的金纳米粒子解决方案可在商业上获得。对于不需要大批量、高单分散度和大直径金纳米粒子的应用，从供应商那里购买和使用这些预合成的金纳米粒子可能就足够了。然而，储存在溶液中的金纳米粒子，如许多市售的纳米粒子，随着纳米粒子开始聚集并形成聚类，可能会随着时间的推移而降解。或者，对于大型应用，需要频繁或长期使用金纳米粒子的长期项目，或对金纳米粒子的单一性和尺寸有更严格的要求的项目，最好自己进行金纳米粒子合成。通过执行金纳米粒子合成过程，我们有机会控制各种合成参数，如所生产的金纳米粒子的数量、金纳米粒子的直径、金纳米粒子的单分性以及用作封盖配体的分子。此外，这种金纳米粒子可以作为固体颗粒储存在干燥的环境中，有助于保存金纳米粒子，以便它们可以在以后的时间，长达一年后，在质量上降解最小。此外，通过大量制造金纳米粒子，然后将其储存在干燥状态，使其持续更长时间，也有可能节省成本和减少浪费。总的来说，合成金纳米粒子本身提供了令人信服的优势，可能不可行与市售的金纳米粒子。
为了实现金纳米粒子合成的诸多优势，本文提出了金纳米粒子合成工艺。所描述的金纳米粒子合成过程是由平松和奥斯特洛12开发的过程的修改版本。黄金纳米粒子通常使用这种合成过程合成，直径约12纳米。用于执行金纳米粒子合成过程的主要化学试剂是四氯酸（HAuCl4）、油胺和甲苯。氮手套箱用于为金纳米粒子合成过程提供惰性干燥环境，因为四氯酸对水/湿度敏感。金纳米粒子用油胺脂蛋白分子封装，以防止金纳米粒子在溶液中聚集。在合成过程结束时，金纳米粒子在真空环境中干燥，以便它们可以储存和保存在干燥状态下供以后使用，最晚一年后使用。当金纳米粒子准备使用时，它们可以再注入溶液中的有机溶剂，如甲苯。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>50 mL Conical Centrifuge Tubes with Plastic Caps (Quantity: 12)</td>
			<td>Ted Pella, Inc.</td>
			<td>12942</td>
			<td>used for cleaning/storing gold nanoparticle solution/precipitate (it&#39;s best to use 12 tubes, to allow the gold nanoparticles from the synthesis process to last up to one year (e.g., 1 tube per month))</td>
		</tr>
		<tr>
			<td>Acetone</td>
			<td>Sigma-Aldrich</td>
			<td>270725-2L</td>
			<td>solvent for cleaning glassware/tubes</td>
		</tr>
		<tr>
			<td>Acid Wet Bench</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>for cleaning chemical reaction glassware/supplies with gold etchant solution (part of wet chemical lab facilities)</td>
		</tr>
		<tr>
			<td>Aluminum Foil</td>
			<td>Reynolds</td>
			<td>B08K3S7NG1</td>
			<td>for covering glassware after cleaning it to keep it clean</td>
		</tr>
		<tr>
			<td>Burette Clamps</td>
			<td>Fisher Scientific</td>
			<td>05-769-20</td>
			<td>for holding the condenser tube and reaction vessel during the synthesis process (located in the nitrogen glove box)</td>
		</tr>
		<tr>
			<td>Centrifuge (with 50 mL Conical Centrifuge Tube Rotor/Adapter)</td>
			<td>ELMI</td>
			<td>CM-7S</td>
			<td>for spinning the gold nanoparticles in solution and precipitating/collecting them at the bottom of the 50 mL conical centrifuge tubes</td>
		</tr>
		<tr>
			<td>DI Water</td>
			<td>Millipore</td>
			<td>Milli-Q Direct</td>
			<td>deionized water</td>
		</tr>
		<tr>
			<td>Fume Hood</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>for cleaning laboratory glassware and supplies with solvents (part of wet chemical lab facilities)</td>
		</tr>
		<tr>
			<td>Glass Beaker (600 mL)</td>
			<td>Ted Pella, Inc.</td>
			<td>17327</td>
			<td>for holding reaction vessel, condenser tube, glass pipette, and magnetic stir bar during cleaning with gold etchant and then with water</td>
		</tr>
		<tr>
			<td>Glass Beakers (400 mL) (Quantity: 2)</td>
			<td>Ted Pella, Inc.</td>
			<td>17309</td>
			<td>for measuring toluene and gold etchant</td>
		</tr>
		<tr>
			<td>Glass Graduated Cylinder (5 mL)</td>
			<td>Fisher Scientific</td>
			<td>08-550A</td>
			<td>for measuring toluene and oleylamine for injection</td>
		</tr>
		<tr>
			<td>Glass Graduated Pipette (10 mL)</td>
			<td>Fisher Scientific</td>
			<td>13-690-126</td>
			<td>used with the rubber bulb with valves to inject the gold nanoparticle precursor solution into the reaction vessel</td>
		</tr>
		<tr>
			<td>Gold Etchant TFA</td>
			<td>Sigma-Aldrich</td>
			<td>651818-500ML</td>
			<td>(with potassium iodide) for cleaning reaction vessel, condenser tube, magnetic stir bar, glass pipette [alternatively, use Aqua Regia]</td>
		</tr>
		<tr>
			<td>Isopropanol</td>
			<td>Sigma-Aldrich</td>
			<td>34863-2L</td>
			<td>solvent for cleaning glassware/tubes</td>
		</tr>
		<tr>
			<td>Liebig Condenser Tube (~500 mm) (24/40)</td>
			<td>Fisher Scientific</td>
			<td>07-721C</td>
			<td>condenser tube, attaches to glass reaction vessel</td>
		</tr>
		<tr>
			<td>Magnetic Stirring Bar</td>
			<td>Fisher Scientific</td>
			<td>14-513-51</td>
			<td>for stirring reaction solution during the synthesis process</td>
		</tr>
		<tr>
			<td>Methanol (&#8805;99.9%)</td>
			<td>Sigma-Aldrich</td>
			<td>34860-2L-R</td>
			<td>new, &#8805;99.9% purity (for washing gold nanoparticles after synthesis)</td>
		</tr>
		<tr>
			<td>Microbalance (mg resolution)</td>
			<td>Accuris Instruments</td>
			<td>W3200-120</td>
			<td>for weighing tetrachloroauric acid powder (located in the nitrogen glove box)</td>
		</tr>
		<tr>
			<td>Micropipette (1000 &#181;L)</td>
			<td>Fisher Scientific</td>
			<td>FBE01000</td>
			<td>for measuring and dispensing liquid chemicals such as oleylamine and toluene (if using micropipette instead of graduated cylinder for measurement)</td>
		</tr>
		<tr>
			<td>Micropipette Tips (1000 &#181;L)</td>
			<td>USA Scientific</td>
			<td>1111-2831</td>
			<td>for measuring and dispensing liquid chemicals such as oleylamine and toluene (if using micropipette instead of graduated cylinder for measurement)</td>
		</tr>
		<tr>
			<td>Nitrile Gloves</td>
			<td>Ted Pella, Inc.</td>
			<td>81853</td>
			<td>personal protective equipment (PPE), for protection, and for keeping nitrogren glove box gloves clean</td>
		</tr>
		<tr>
			<td>Nitrogen Glove Box</td>
			<td>M. Braun</td>
			<td>LABstar pro</td>
			<td>for performing gold nanoparticle synthesis in a dry and inert environment</td>
		</tr>
		<tr>
			<td>Non-Aqueous 20 mL Glass Vials with PTFE-Lined Caps (Quantity: 2)</td>
			<td>Fisher Scientific</td>
			<td>03-375-25</td>
			<td>for weighing tetrachloroauric acid powder and mixing with oleylamine and toluene to make injection solution</td>
		</tr>
		<tr>
			<td>Oleylamine (Technical Grade, 70%)</td>
			<td>Sigma-Aldrich</td>
			<td>O7805-100G</td>
			<td>technical grade, 70%, preferably new, stored in the nitrogen glove box</td>
		</tr>
		<tr>
			<td>Parafilm M Sealing Film (2 in. x 250 ft)</td>
			<td>Sigma-Aldrich</td>
			<td>P7543</td>
			<td>for sealing the gold nanoparticles in the 50 mL centrifuge tubes after the synthesis process is over</td>
		</tr>
		<tr>
			<td>Round Bottom Flask (250 mL) (24/40)</td>
			<td>Wilmad-LabGlass</td>
			<td>LG-7291-234</td>
			<td>glass reaction vessel, attaches to condenser tube</td>
		</tr>
		<tr>
			<td>Rubber Bulb with Valves (Rubber Bulb-Type Safety Pipet Filler)</td>
			<td>Fisher Scientific</td>
			<td>13-681-50</td>
			<td>used with the long graduated glass pipette to inject the gold nanoparticle precursor solution into the reaction vessel</td>
		</tr>
		<tr>
			<td>Rubber Hoses (PVC Tubes) (Quantity: 2)</td>
			<td>Fisher Scientific</td>
			<td>14-169-7D</td>
			<td>for connecting the condenser tube to water inlet/outlet ports</td>
		</tr>
		<tr>
			<td>Stainless Steel Spatula</td>
			<td>Ted Pella, Inc.</td>
			<td>13590-1</td>
			<td>for scooping tetrachloroauric acid powder from small container</td>
		</tr>
		<tr>
			<td>Stand (Base with Rod)</td>
			<td>Fisher Scientific</td>
			<td>12-000-102</td>
			<td>for holding the condenser tube and reaction vessel during the synthesis process (located in the nitrogen glove box)</td>
		</tr>
		<tr>
			<td>Stirring Heating Mantle (250 mL)</td>
			<td>Fisher Scientific</td>
			<td>NC1089133</td>
			<td>for holding and supporting reaction vessel sphere, while heating with magnetic stirrer rotating the magnetic stirrer bar</td>
		</tr>
		<tr>
			<td>Tetrachloroauric(III) Acid (HAuCl4) (&#8805;99.9%)</td>
			<td>Sigma-Aldrich</td>
			<td>520918-1G</td>
			<td>preferably new or never opened, &#8805;99.9% purity, stored in fridge, then opened only in the nitrogen glove box, never exposed to air/water/humidity</td>
		</tr>
		<tr>
			<td>Texwipes / Kimwipes / Cleanroom Wipes</td>
			<td>Texwipe</td>
			<td>TX8939</td>
			<td>for miscellaneous cleaning and surface protection</td>
		</tr>
		<tr>
			<td>Toluene (&#8805;99.8%)</td>
			<td>Sigma-Aldrich</td>
			<td>244511-2L</td>
			<td>new, anhydrous, &#8805;99.8% purity</td>
		</tr>
		<tr>
			<td>Tweezers</td>
			<td>Ted Pella, Inc.</td>
			<td>5371-7TI</td>
			<td>for poking small holes in aluminum foil, and for removing Parafilm</td>
		</tr>
		<tr>
			<td>Vortexer</td>
			<td>Cole-Parmer</td>
			<td>EW-04750-51</td>
			<td>for vortexing the gold nanoparticles in toluene in 50 mL conical centrifuge tubes to resuspend the gold nanoparticles into the toluene solution</td>
		</tr>
	</tbody>
</table>

gold nanoparticle synthesis

直径约12纳米的金纳米粒子（Au纳米粒子）通过在3.0克（3.7 mmol）中快速注入150毫克（0.15 mmol）的四氯酸溶液进行合成， 3.6 mL）的油胺（技术等级）和3.0 mL的甲苯在147 mL的甲苯中放入5.1克（6.4毫米醇，8.7毫升）的沸腾溶液中。在煮沸和混合反应溶液2小时时，反应混合物的颜色从透明变为浅黄色，变为浅粉色，然后慢慢变为深红色。然后关闭热量，并允许溶液逐渐冷却到室温1小时。然后，用离心机收集金纳米粒子并与溶液分离，洗三次：通过在10mL的甲苯部分旋转和分散金纳米粒子，然后通过加入40mL的甲醇部分并在离心机中旋转它们来沉淀金纳米粒子。然后，解决方案被拆除，以删除任何剩余的副产品和未经反应的起始材料。在真空环境中干燥金纳米粒子产生固体黑颗粒：可长期储存（最多一年）供以后使用，然后重新溶解在有机溶剂中，如甲苯。

图1显示金纳米粒子合成化学反应混合物溶液（四氯酸、油胺和甲苯）在反应容器中最初沸腾时，应在几分钟内逐渐改变颜色：从透明到浅黄色（左图），到浅粉色（中心图像），到浅红色（右图）。溶液颜色的变化表明，随着金纳米粒子开始核化并随着时间的推移而变大，其大小也在不断变化。一般来说，随着金纳米粒子的核化和生长，金纳米粒子溶液应随着时间的推移变...

Watch this Scientific Journal Video about Gold Nanoparticle Synthesis at JoVE.com

Gold Nanoparticle Synthesis

提出了在有机溶剂中合成直径约12纳米的金纳米粒子（Au纳米粒子）的协议。金纳米粒子用油胺脂蛋白封顶，以防止聚集。金纳米粒子可溶于甲苯等有机溶剂中。

金纳米粒子合成

Gold nanoparticles (Au nanoparticles) that are ~12 nm in diameter were synthesized by rapidly injecting a solution of 150 mg (0.15 mmol) of ...

这种金纳米粒子合成工艺的目标是制造直径约12 nm.in、非常单分的金纳米粒子。这种金纳米粒子合成过程是平松弘一和弗兰克·奥斯特洛在研究文章中描述的工艺的修改版本。 合成和纯化、四氯酸、油胺、甲苯和甲醇所需的化学品。开始设置和清洁化学反应玻璃器皿和氮手套箱中的用品。确保磁搅拌棒位于玻璃反应容器内。将大约200毫升的甲苯倒入玻璃反应容器中。将带甲苯的玻璃反应容器放在搅拌加热的地衣上，并将凝结管送入反应容器。慢慢打开并监测水，以确保水轻轻地流入凝结管。通过稍微打开或关闭水阀，根据需要调整水流。允许水流经凝结管底部的入口端口、凝结管上，以及凝结管顶部的出水口。不断将新鲜氮气流入手套箱，以清除手套箱。通过在手套箱上拉一个轻微的真空来持续通风手套箱。开始加热，并在搅拌和加热地衣上用磁搅拌器搅拌甲苯。让甲苯接近一个温柔的沸腾。不要接近或超过甲苯的闪光点。开始沸腾时减少热量。让甲苯沸腾并蒸发30分钟，以清洁反应玻璃器皿。蒸发的甲苯会在冷凝管中冷却和凝结，然后滴回反应容器中。30分钟后，关闭加热器，让甲苯冷却几分钟，直到甲苯停止蒸发。甲苯冷却后，抬起冷凝管，用夹子将冷凝管悬挂在反应容器上方。将反应容器中的甲苯倒入 400 ml.beaker.Be 小心不要意外地倒出磁搅拌棒。将反应容器放回加热和搅拌地衣上。将甲苯旋转到 400 毫升玻璃烧杯中，以清洁烧杯。将脏/用过的甲苯倒入易燃废水瓶中。用一些新鲜的甲苯再次清洁烧杯，然后丢弃用过的甲苯进入易受攻击的废瓶中。在 8.7 毫升或约 9 毫升油胺时，制作 147 毫升或约 150 毫升的甲苯沸腾溶液。使用 400 毫升玻璃烧杯测量 147 毫升甲苯。将玻璃烧杯中的 147 毫升甲苯倒入反应容器中。注意：奥莱胺是有毒和腐蚀性的，所以小心处理它。不要泄漏油胺使用5毫升小玻璃分级气瓶仔细测量和倒入8.7毫升油胺。例如，先将 4 毫升，然后倒入 4.7 毫升，小心地将玻璃分级圆柱体中的油胺倒入反应容器中。再次将玻璃凝结管降低到玻璃反应容器中。确保水流经冷凝管的外室冷却、凝结和收集甲苯和油胺蒸汽。加热和搅拌溶液，使溶液接近温和沸腾。开始准备注射液：150毫克四氯酸，3.6毫升油胺和3毫升甲苯。取出保护四氯酸免受空气和水分的实验室膜或密封。四氯酸对水、水分和湿度非常敏感。应尽一切努力防止四氯酸粉暴露在空气和水中。四氯酸容器应只打开并储存在氮手套箱中。使用金属铲将四氯酸粉从容器中沉积到微平衡玻璃瓶中，测量重量为150毫克的四氯酸粉末。注意：小心处理油胺，因为它是有毒和腐蚀性的。使用 5 毫升小玻璃分级圆柱体测量 3.6 毫升油胺。小心地将 5 毫升小玻璃分级圆柱体中的 3.6 毫升油胺倒入 20 毫升玻璃瓶中，不含有四氯酸。小心地将 3 毫升甲苯倒入 5 毫升小玻璃分级圆柱体中。小心地将 5 毫升小玻璃分级圆柱体中的 3 毫升甲苯与油胺一起倒入 20 毫升玻璃瓶中。将 PTFE 衬里帽拧回 20 毫升玻璃瓶上，里面有油胺和甲苯。摇动和旋转封闭的玻璃瓶，将奥莱兰明和甲苯溶液混合在一起。小心地将150毫克的四氯酸粉末与油胺和甲苯溶液一起倒入玻璃瓶中。将 PTFE 衬里帽拧回玻璃瓶上。用四氯酸、油胺和甲苯摇动和旋转封闭的玻璃瓶，将溶液混合在一起。不断摇动解决方案，并确保它彻底混合。四氯酸、油胺和甲苯溶液应变深红色。确保水缓慢流入凝结管底部和凝结管顶部。通过小心打开或关闭水阀，根据需要调整水流。确保反应容器中的油胺和甲苯溶液处于温和沸腾，一些甲苯和油胺蒸发到凝结管中。确保磁搅拌器处于打开。用夹子将凝结管举到反应容器上方，用夹子支撑玻璃器皿。在使用橡胶灯泡和玻璃管帽之前，请确保您知道如何操作橡胶灯泡。挤压橡胶灯泡以放气橡胶灯泡时按压上部阀门。小心地将长分级玻璃移液器的尖端放入 20 毫升玻璃瓶中，并加入四氯酸、油胺和甲苯注射液。轻轻按压橡胶球上的下阀，将四氯酸、油胺和甲苯注射液慢慢吸入玻璃移液器中。小心地将玻璃移液器的尖端放入反应容器的开口中，并迅速将四氯酸、油胺和甲苯溶液注入反应容器中。颜色应在大约一分钟内从红色变为黄色变为白色，因为金纳米粒子开始核化和生长。将凝结管向下降低到反应容器中。将溶液轻轻煮沸两小时。在几分钟内，随着金纳米粒子变大，颜色应从白色变为黄色，从粉红色变为红色。几分钟后，溶液变成粉红色或浅红色。几分钟后，解决方案更加红。溶液在沸腾两小时后将继续变深变深。加热两小时后，关闭加热器。让金纳米粒子溶液自然冷却一小时。确保加热器关闭，解决方案冷却。停止流水通过凝结管。甲醇将用于清洁金纳米颗粒，去除未经反应的化学试剂和副产品。将35毫升甲醇倒入50毫升的离心机管中。然后将12毫升的金纳米粒子溶液倒入50毫升的锥形离心机管中，加入甲醇。小心不要不小心倒出磁搅拌棒，同时将金纳米粒子溶液倒入每个离心机管中。将12毫升的金纳米粒子溶液倒入50毫升的圆锥离心管和35毫升甲醇后，每个离心管应有约47毫升的溶液。在离心机管之间均匀地分配剩余的金纳米粒子溶液。将盖子拧到 50 毫升离心管上，以关闭它们并拧紧盖子。从氮手套箱中取出50毫升圆锥形离心管，配上金色纳米粒子溶液和甲醇。离心管应具有同等数量的金纳米粒子溶液，并且应具有同等的加权和平衡。将50毫升离心管中的6个与金纳米粒子放入离心机。关闭离心机的盖子，进入旋转金纳米粒子的设置。RCF：1000.时间：5分钟。开始旋转离心机。前六根带金纳米粒子的离心管旋转后，轻轻地从离心机中取出。小心不要打扰金纳米粒子颗粒，并将管子放在机架上。离心力将拉下溶液中的金纳米粒子，并将它们从甲醇和甲苯中分离。金纳米粒子会沉淀到每个离心机管底部的颗粒中。将最后六根带金纳米粒子的离心管放入离心机中。关闭离心机的盖子，进入旋转金纳米粒子的设置。RCF：1000.时间：5分钟。开始旋转。离心管旋转后，轻轻地从离心机中取出，放在离心机架上。小心地将所有离心管带到烟罩上。慢慢地轻轻地将每个离心管中的溶液倒入易燃废物中，放入烧瓶中。小心不要打扰，不要倒出管子底部的金纳米粒子颗粒。第一个甲醇冲洗周期现已完成。开始第二个甲醇冲洗周期，将10毫升的甲苯倒入每个离心管。涡流每个离心管，以恢复和分散金纳米粒子在整个10毫升。甲苯溶液。检查离心管底部，确保黑色残留物已重新使用。将14毫升甲醇倒入每个离心管中。由于每个离心管中已有10毫升的甲苯，每根离心管将有50毫升的溶液。将盖子拧回 50 毫升离心管上，确保盖子紧绷。在离心机中旋转离心管，将金纳米粒子收集到每个管底部的颗粒中。使用与以前相同的离心机设置。小心地将甲醇和甲苯倒入易燃废物中，像以前一样烧嘴。第二个甲醇冲洗周期现已完成。对于第三个也是最后一个冲洗周期，遵循与以前相同的过程，在甲醇中旋转，清洗甲醇，离心，并小心地倒出溶剂。在倒出最后一个甲苯和甲醇冲洗溶液后，需要干燥金纳米粒子。建议的程序是真空干燥金纳米粒子。松开 50 毫升离心管上的盖子，使管子仍然覆盖，但溶剂可以蒸发并从管内逸出。将带金纳米粒子的管架放在真空负载锁内。关闭并密封外负载锁门，打开真空泵的阀门，开始拉动负载锁上的真空。泵真空，直到达到中等真空压力，大约在真空压力表指示器的中间。将金色纳米粒子留在负载锁中，在大约半仪表的中度真空压力下保持几分钟。不要泵到较低的压力，不要在真空中离开太久，否则配体可能会分离。金纳米粒子在真空中干燥金纳米粒子并蒸发剩余溶剂后，用氮清除负载锁，直到负载达到大气压力。从负载锁中取出带有金纳米粒子的 50 毫升离心管，并检查烟罩内金纳米颗粒的干燥程度。金纳米粒子颗粒干燥后，将盖子拧回离心管上。将实验室膜包裹在紧密封闭的盖子上，用金色的纳米粒子颗粒密封离心机管。用金纳米粒子标记离心机管，并将其放置在 2 中

Research

JoVE Journal

Chemistry

Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance

的模板导演电浆黄金纳米管的合成与可调谐红外吸收

A nearly parallel array of pores can be produced by anodizing aluminum foils in acidic environments1, 2. Applications of anodic aluminum oxide (AAO) ...

科研

化学

Synthesis and Catalytic Performance of Gold Intercalated in the Walls of Mesoporous Silica

合成和金插在介孔二氧化硅的墙壁催化性能

As a promising catalytically active nano reactor, gold nanoparticles intercalated in mesoporous silica (GMS) were successfully synthesized and properties ...

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers

光动力疗法与混合导电高分子/富勒烯纳米颗粒光敏剂

In this article a method for the fabrication and reproducible in-vitro evaluation of conducting polymer nanoparticles blended with fullerene as the next ...

A Simple Method for the Size Controlled Synthesis of Stable Oligomeric Clusters of Gold Nanoparticles under Ambient Conditions

根据环境条件的金纳米粒子的稳定低聚簇的大小控制合成的一种简便方法

Reducing dilute aqueous HAuCl4 with sodium thiocyanate (NaSCN) under alkaline conditions produces 2 to 3 nm diameter nanoparticles. Stable grape-like ...

Hydroquinone Based Synthesis of Gold Nanorods

金纳米棒基于对苯二酚合成

Gold nanorods are an important kind of nanoparticles characterized by peculiar plasmonic properties. Despite their widespread use in nanotechnology, the ...

Synthesis and Testing of Supported Pt-Cu Solid Solution Nanoparticle Catalysts for Propane Dehydrogenation

用于丙烷脱氢的负载型Pt-Cu固溶体纳米粒子催化剂的合成与测试

A convenient method for the synthesis of bimetallic Pt-Cu catalysts and performance tests for propane dehydrogenation and characterization are ...

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

用于跟踪纳米粒子自组装的液体细胞透射电子显微镜

Drying a nanoparticle dispersion is a versatile way to create self-assembled structures of nanoparticles, but the mechanism of this process is not fully ...

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

原位金纳米粒子合成快速 Nanoprobe 信号增强

The use of nanoprobes such as gold, silver, silica or iron-oxide nanoparticles as detection reagents in bioanalytical assays can enable high sensitivity ...

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism

通过活性表面生长机制合成基片束缚金纳米线

Advancing synthetic capabilities is important for the development of nanoscience and nanotechnology. The synthesis of nanowires has always been a ...

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

两栖金纳米粒子的合成与表征

Gold nanoparticles covered with a mixture of 1-octanethiol (OT) and 11-mercapto-1-undecane sulfonic acid (MUS) have been extensively studied because of ...