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DOI :
10.3791/62179-v
•
9:26 min
March 23rd, 2021
Chapters
0:04
Introduction
0:26
Tissue Crosslinking
2:24
Chromatin Isolation
3:26
DNA Purification
6:50
Results: ChIP‐qPCR and ChIP‐Seq Analysis
8:52
Conclusion
このプロトコルは、スナップ凍結組織からのクロマチン調製に焦点を当てており、架橋クロマチン免疫沈降(X-ChIP)とそれに続く定量的PCR分析(X-ChIP-qPCR)または次世代シーケンシングアプローチ(X-ChIP-seq)のいずれかに適しています。
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