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DOI :
10.3791/62219-v
July 10th, 2021
Chapters
0:04
Introduction
1:21
Parasite Cultures
2:00
Control Immunofluorescence Protocol
3:23
Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host
4:18
Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species
5:16
Confocal Imaging Acquisition
5:39
Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence
6:51
Conclusion
ここで、プロトコルは、宿主と病原体の相互作用を研究するために同じ種で生起される一次抗体を用いて二重標識免疫蛍光を行う方法を説明する。また、このプロトコルには、別の宿主からの第3の抗体を含むことができる。このアプローチは、任意の細胞型および病原体で行うことができる。
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