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DOI :
10.3791/62296-v
•
7:40 min
April 5th, 2021
Chapters
0:04
Introduction
0:52
Sample Preparation
1:58
Lightsheet Microscope Setup
4:36
Image Analysis
6:23
Results: Time-Lapse Imaging of Early Eye Development in Zebrafish
7:17
Conclusion
在这里,提供了一种方案,用于使用市售的光照相显微镜和图像处理工作站对眼部形态发生进行延时成像,以分析结果数据。该协议详细说明了胚胎麻醉的程序,嵌入低熔点温度琼脂糖,悬浮在成像室中,设置成像参数,最后使用图像分析软件分析成像数据。
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