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DOI :
10.3791/62321-v
•
6:10 min
April 8th, 2021
Chapters
0:04
Introduction
0:47
Preparation of Negatively Stained Samples with Amorphous Carbon Support
2:21
Preparation of Graphene Oxide-coated TEM Grids
3:27
Preparation of Samples on Monolayer-graphene Films Using the Flotation Block
4:44
Results: Examples of Sample Support Films Prepared Using the Floatation Block
5:34
Conclusion
冷冻电子显微镜(cryo-EM)的样品制备是该方法结构测定工作流程中的一个重大瓶颈。在这里,我们提供了使用易于使用的三维打印块制备支撑膜的详细方法,以稳定样品以进行透射EM研究。
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