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DOI :
10.3791/62321-v
•
6:10 min
April 8th, 2021
Chapters
0:04
Introduction
0:47
Preparation of Negatively Stained Samples with Amorphous Carbon Support
2:21
Preparation of Graphene Oxide-coated TEM Grids
3:27
Preparation of Samples on Monolayer-graphene Films Using the Flotation Block
4:44
Results: Examples of Sample Support Films Prepared Using the Floatation Block
5:34
Conclusion
この方法の構造決定ワークフローにおいて、クライオ電子顕微鏡(cryo-EM)のサンプル調製は重要なボトルネックとなっている。ここでは、透過EM試験用サンプルを安定化させる支持膜の作製に使いやすい3次元印刷ブロックを用いた詳細な方法を提供する。
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