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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

DOI :

10.3791/62395-v

April 19th, 2021

April 19th, 2021

4,364 Views

1Department of Chemistry, Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University

Here, a detailed description of the protocol implemented in the laboratory for acquisition and analysis of 15N relaxation dispersion profiles by solution NMR spectroscopy is provided.

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