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DOI :
10.3791/62398-v
February 14th, 2022
Chapters
0:04
Introduction
0:38
Deuterium (D2O) Incorporation Treatment in the Presence of Antibiotics
3:50
Preparation of Bacteria in Urine Environment and Blood Environment
5:42
Stimulated Raman Scattering (SRS) Imaging of D2O Metabolic Incorporation in a Single Bacterium
8:44
Results: The Influence of Deuterium Incorporation on Bacterial Growth
11:14
Conclusion
이 프로토콜은D2O대사의 단일 세포 자극 라만 산란 이미징에 의해 2.5시간 이내에 신속한 항균 감수성 테스트(AST) 분석을 제공합니다. 이 방법은 소변 또는 전혈 환경의 박테리아에 적용되며, 이는 클리닉에서 신속한 단일 세포 표현형 AST를 변형시킵니다.
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