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Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing (Video) | JoVE

DOI :

10.3791/62417-v

7:27 min

March 26th, 2021

March 26th, 2021

1,926 Views

1Department of Entomology and Plant Pathology, North Carolina State University

Herein are protocols for collecting and microinjecting precellular corn planthopper embryos for the purpose of modifying their genome via CRISPR/Cas9-based genome editing or for the addition of marked transposable elements through germline transformation.

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Microinjection

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