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DOI :
10.3791/62417-v
•
7:27 min
March 26th, 2021
Chapters
0:04
Introduction
1:08
Preparation of Agarose‐Based Egg‐Lay Chamber
2:23
Embryo Collection and Alignment
2:59
Microinjection and Post‐Injection Care
3:48
Incubation and Hatching of Embryos
5:03
Results: Microinjection of P. maidis Embryos for CRISPR/Cas9 Genome Editing and Collection
6:32
Conclusion
以下是收集和微注射细胞前玉米飞虱胚胎的方案,目的是通过基于CRISPR / Cas9的基因组编辑修改其基因组或通过种系转化添加标记的转座元件。
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