Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/62417-v
•
7:27 min
March 26th, 2021
Chapters
0:04
Introduction
1:08
Preparation of Agarose‐Based Egg‐Lay Chamber
2:23
Embryo Collection and Alignment
2:59
Microinjection and Post‐Injection Care
3:48
Incubation and Hatching of Embryos
5:03
Results: Microinjection of P. maidis Embryos for CRISPR/Cas9 Genome Editing and Collection
6:32
Conclusion
여기에서는 CRISPR/Cas9 기반 게놈 편집을 통해 게놈을 수정하거나 생식계열 형질전환을 통해 표시된 전치 요소를 추가할 목적으로 세포 전 옥수수 식물 호퍼 배아를 수집하고 미세 주입하기 위한 프로토콜입니다.
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved