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DOI :
10.3791/62432-v
•
7:10 min
May 7th, 2021
Chapters
0:04
Introduction
0:41
Preparation of Male and Female Mosquitoes
1:11
Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
1:52
Checking for RhB Fluorescence in Male Mosquitoes
2:46
Mating Competitiveness Assay
5:36
Results: Assessment of Mating Competitiveness in Male Mosquitoes
6:27
Conclusion
ここでは、蛍光色素をマーカーとして用いた雄 のAedes aegypti の交配性を研究するためのプロトコルを提示する。雌の蚊は、交尾のためにマークされた男性と無印の両方の雄にさらされます。交配後、それらの精子は、交配パートナーを決定するために蛍光顕微鏡下で検査される。
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