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DOI :
10.3791/62452-v
April 13th, 2021
Chapters
0:04
Introduction
0:59
Setting up the Kidney Organoid Assay and Feeding by Half-Medium Change
2:49
Transfer of Embryoid Bodies to Stage II Medium
4:49
Transfer to Spinner Flask and Feeding
5:37
Setting up a 6-Well Magnetic Stir Plate
6:14
Results: Immunofluorescence of the Kidney Organoids
6:51
Conclusion
ここでは、ヒト多能性幹細胞(hPSC)から腎臓オルガノイドを生成するためのプロトコルについて説明する。このプロトコルは、2週間以内に腎臓オルガノイドを生成する。得られた腎臓オルガノイドは、大規模なスピナーフラスコまたはマルチウェル磁気攪拌プレートで培養して、薬物試験アプローチを並行させることができます。
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