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DOI :
10.3791/62469-v
•
6:26 min
May 16th, 2021
Chapters
0:04
Introduction
0:35
Plate Preparation
1:06
Fragment Nano-Dispensing by the Mosquito Robot
2:01
Fragment Dispensing
3:17
Preparing the Prometheus
4:25
Fluorescence Scan and Thermal Denaturation
5:04
Results: Fragment Screening
5:49
Conclusion
监测目标蛋白(即热移位检测、TSA)融化温度的变化是筛选几百种化合物片段库的有效方法。我们提出了一个TSA协议,实施机器人辅助纳米微分扫描荧光仪(nano-DSF),用于监测内在色氨酸荧光和光反散射,用于碎片筛选。
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