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DOI :
10.3791/62554-v
May 11th, 2021
Chapters
0:04
Introduction
1:14
Embryoid Bodies (EB) Formation in Hanging Drop
3:02
Floating EBs Culture for Vascular Differentiation
3:41
3D Sprouting Angiogenesis Assay and Immunofluorescence Staining
5:44
Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay
7:29
Conclusion
该测定利用分化成在3D胶原蛋白凝胶中培养的拟胚体的小鼠胚胎干细胞来分析体 外控制发芽血管生成的生物学过程。该技术可用于测试药物,模拟疾病以及在胚胎致命的缺失背景下研究特定基因。
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