JoVE Logo
Faculty Resource Center

Sign In

Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

DOI :

10.3791/62568-v

June 9th, 2021

June 9th, 2021

2,205 Views

1Department of Neurobiology, Duke University Medical Center

Here we describe a method to visualize synaptogenesis of granule neurons in the mouse cerebellum over the time course of postnatal brain development when these cells refine their synaptic structures and form synapses to integrate themselves into the overall brain circuit.

-- Views

Related Videos

article

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

article

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

article

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

article

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

article

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

article

Laser Nanosurgery of Cerebellar Axons In Vivo

article

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

article

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

article

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

article

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved