Preparing the Injection Syringe for Electroporation
1:00
Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
2:34
Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
3:45
Conclusion
Transcript
The protocol studies cerebellar granule neurons across different stages of development in order to visualize key morphological growth. The advantages of the technique include cell specific targeting, fast expression of transfected constructs, and
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Here we describe a method to visualize synaptogenesis of granule neurons in the mouse cerebellum over the time course of postnatal brain development when these cells refine their synaptic structures and form synapses to integrate themselves into the overall brain circuit.