Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
11.6K Views
•
08:53 min
•
May 10th, 2017
DOI :
May 10th, 2017
•Lin Jiajia*1, Mak Shinghung*2, Zheng Jiacheng1, Wang Jialing1, Xu Dilin1, Hu Shengquan2, Zhang Zaijun3, Wang Qinwen1, Han Yifan2,4, Cui Wei1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Explore More Videos
Chapters in this video
0:05
Title
0:46
Tissue Harvest from 8 Day Old Sprague-Dawley Rats
3:51
FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
5:30
Assessment of Neuronal Viability
7:08
Results: Double and Triple Stainings in CGN Culture
8:19
Conclusion
Related Videos
09:02
Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
15.1K Views
08:10
Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities
21.0K Views
09:10
Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
9.3K Views
10:36
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
8.0K Views
Live Animal Imaging and Cell Sorting Methods for Investigating Neurodegeneration in a C. elegans Excitotoxic Necrosis Model
1.8K Views
A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution
967 Views
01:30
Fluorescence Immunostaining of Cerebellar Organoid Slices
36 Views
01:27
Assessing Neuronal Viability in a Cerebellar Neuron Culture via Double Staining
46 Views
01:26
Modeling Neuronal Injury in Mouse Primary Cerebellar Granule Neurons
46 Views
01:24
Modeling Reactive Oxygen Species-Induced Neuronal Death in Mouse Cerebellar Granule Neurons
43 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved