Assessment of Phagocytic Function by Flow Cytometry
1:17
Assessment of Lymphocyte Function in Peripheral Blood
2:30
Assessment of Lymphocyte Function/ Inflammatory Mediators in Lung Tissue
4:16
Assessment of Lung Proteolysis by Confocal Microscopy
4:45
Results: ROS and Cytokine Production by Monocytes and In Situ Zymography of the Lung Tissue
5:52
Conclusion
Transcript
Haemophilus influenza is a major cause of inflammation in various chronic lung diseases including COPD and pneumonia. This protocol describes methods for assessing the effect of haemophilus on lung inflammation. The advantages of this technique is
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Haemophilus influenzae induces inflammation in the respiratory tract. This article will focus on the use of flow cytometry and confocal microscopy to define immune responses by phagocytes and lymphocytes in response to this bacterium.