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DOI :
10.3791/62607-v
•
5:51 min
June 28th, 2021
Chapters
0:04
Introduction
0:48
PCR Preparation and Droplet Generation
2:18
PCR
3:03
Droplet Reading
3:31
Analysis
4:49
Results: Implications of ddPCR for NHEJ Identification and Quantification in Transgenic Anopheline Mosquitoes
5:27
Conclusion
このプロトコルは、gRNA誘導Cas9切断およびDNA修復後の標的部位における非相同性端結合(NHEJ)事象の同定および定量化に関する分析を含む、DNA抽出からデジタル液滴PCR(ddPCR)の実験的セットアップまでのステップを提供する。この方法の他の用途としては、多型検出や遺伝子編集バリアント検証などの用途が挙げられる。
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