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DOI :
10.3791/62608-v
September 14th, 2021
Chapters
0:05
Introduction
0:40
Cell Culture and Fluid Shear Stress Exposure
2:55
Fixation
4:58
Blocking and Incubation with Primary Antibody
6:28
Incubation with PLA Probe and Ligation
7:34
Amplification and Mounting
9:14
Results: Effect of Shear Stress on the SMAD Protein Interactions
11:02
Conclusion
在这里,我们建立了一个方案,使用暴露于病理和生理流体剪切应激条件下的内皮细胞中的接近连接测定(PLA)同时可视化和分析多个SMAD复合物。
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