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DOI :
10.3791/62622-v
•
11:51 min
September 22nd, 2021
Chapters
0:04
Introduction
1:29
Lymphocyte Isolation and Alpha-beta (αβ) T Cell Depletion
6:16
Co-culture with Artificial Antigen-Presenting Cells (aAPCs) and Cell Harvest
9:22
Results: Characterization and Optimization of the γδ T Cell Expansion Process
11:21
Conclusion
提示は、ガンマデルタ(γδ)T細胞薬剤産物の拡張のためのプロトコルである。リンパ球は溶出により単離され、γδ濃縮はゾレドロン酸とインターロイキン-2で濃縮される。α-βT細胞は、臨床グレードの磁気分離装置を使用して枯渇する。γδ細胞をK562由来、人工抗原提示細胞と共培養し、拡大する。
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