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DOI :
10.3791/62623-v
•
8:23 min
December 25th, 2021
Chapters
0:00
Introduction
0:44
Preparation of Samples
1:26
Sample Irradiations
1:35
Slide Preparation
2:17
Fixation and Immunofluorescence γ-H2AX Staining
3:44
Automated Metafer Scanning and Scoring
7:29
Results
8:03
Conclusion
该协议介绍了外周血淋巴细胞上γ-H2AX病灶测定的载玻片制备和自动评分。为了说明测定的方法和灵敏度,在 体外照射分离的淋巴细胞。这种自动化的DNA DSB检测方法对于快速和高通量的生物剂量测定应用非常有用。
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