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使用胶原基支架的神经母细胞瘤三维 体外 仿生模型

DOI :

10.3791/62627-v

7:48 min

July 9th, 2021

July 9th, 2021

2,858 Views

1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 4National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland

本文列出了在先前描述的三维胶原基支架上接种神经母细胞瘤细胞系所需的步骤,在预定的时间范围内维持细胞生长,以及检索支架以进行多种细胞生长和细胞行为分析以及下游应用,可适应满足一系列实验目标。

Tags

ECM nHA GAG 2D DNA

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