Name: human subcutaneous adipose tissue sampling using a mini-liposuction technique
Uploaded: 2021-09-27T13:00:00
Description: Watch this Scientific Journal Video about Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique at JoVE.com

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注意：斯特灵大学NHS，侵入性或临床研究委员会批准了下面描述的活检程序。所有使用此程序的研究必须得到适当的独立伦理委员会的批准。活检者必须根据其机构的要求完成所述技术的正式培训。通常，这涉及由董事会认证的医生观察所描述的脂肪组织活检技术的演示，然后进行监督实践。一旦受训者在监督下对志愿者受试者进行了 10 次练习脂肪组织活检，他们将由董事会认证的医生进行检查...

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所描述的方案和相关视频提供了从腹部区域获得皮下脂肪组织样本的微型吸脂技术的分步概述。该研究小组在19个月内共进行了124次活检，对参与者没有不良反应。该程序是安全的，并且对参与者或活检团队的风险最小，前提是遵循所描述的安全措施。执行该程序的研究人员必须始终保持无菌技术（包括打开和分配无菌设备而不污染它们，适当地戴/摘下无菌手套，一般手部卫生）（以尽量减少参?...

脂肪组织可以提供有关人类代谢功能的有用信息。人体皮下脂肪组织很容易获得。皮下脂肪组织提取技术在 80 年代中期首次被描述1;从那时起，初始方案得到了改进，以提高产量并提高研究参与者的耐受性。皮下脂肪组织可以从许多部位获得，最常见的是从臀部1 和腹部区域2获得。来自后者的样本可能更理想，因为它们在代谢疾病相关背景下提供了更有价值的信息3。
使用微型吸脂方法的皮下脂肪组织活检可以在非临床环境中安全有效地进行。经过董事会认证的医生的适当培训并使用严格的无菌技术，研究人员可以常规进行这些活检，对参与者和研究人员的风险最小。活检团队必须由至少 2 人组成：进行活检的人和助手。
负责活检的人员的任务是确认参与者的身份，检查参与者是否可以安全地接受该程序（请参阅下面的协议步骤2.1-2.4），确保参与者在整个过程中感到舒适，确保在整个过程中保持无菌技术，执行程序，并为参与者提供口头和书面的善后程序。助手的作用是处理和快速处理获得的脂肪组织，以便以后分析和/或储存。助手还通过成为“非无菌手”来提供帮助，并确保参与者在整个过程中保持轻松。本视频和论文的目的是描述分步活检程序，以安全地从腹部区域获取皮下脂肪组织。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Item: 14 G needle 
			14 G x 3 1/8&#34; 210 mm x 80 mm</td>
			<td>B Braun</td>
			<td>4665473</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: 21 G needle 
			21 G x 1 1/2&#34; 0.8 mm x 38 mm</td>
			<td>Terumo</td>
			<td>AN*2138R1</td>
			<td>Per biopsy: 2</td>
		</tr>
		<tr>
			<td>Item: 26 G&#160; needle 
			26 G x 1/2&#34; microlance needle 0.45 mm x 13 mm</td>
			<td>BD</td>
			<td>303800</td>
			<td>Per biopsy: 2</td>
		</tr>
		<tr>
			<td>Item: 5 mL syringe 
			5 mL luer</td>
			<td>DB plastipak</td>
			<td>302187</td>
			<td>Per biopsy: 2</td>
		</tr>
		<tr>
			<td>Item: Adhesive wound dressing 
			Opsite Post-Op Dressing 9.5 x 8.5</td>
			<td>Smith &#38; Nephew</td>
			<td>6600709</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: Disposable sterile scalpel 
			Disposable Scalpel Sterile Blade no. 10</td>
			<td>Swann Morton</td>
			<td>/0501</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: Icepack 
			BlueDot Reusable Hot/Cold Pack 26.5 cm x 13.0 cm</td>
			<td>NuCare</td>
			<td>F711</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: Iodine based antiseptic 
			Videne antiseptic solution</td>
			<td>Ecolab Videne</td>
			<td>3030440</td>
			<td>Per biopsy: q.s</td>
		</tr>
		<tr>
			<td>Item: Lidocaine 2% w/o epinephrine 
			Lidocaine 2% injection 5 mL</td>
			<td>B Braun</td>
			<td>3558553</td>
			<td>Per biopsy: 5 mL</td>
		</tr>
		<tr>
			<td>Item: Non-sterile gloves 
			Starguard sensitive powder free nitrile gloves</td>
			<td>Starguard</td>
			<td>SG-N-S</td>
			<td>Per biopsy: pair</td>
		</tr>
		<tr>
			<td>Item: Sodium chloride 0.9% 
			Sodium chlride 0.9% w/v intravenous infusion BP</td>
			<td>BBraun</td>
			<td>S8004-5384</td>
			<td>Per biopsy: q.s.</td>
		</tr>
		<tr>
			<td>Item: Stabilization solution* 
			RNAlater Stabilization Solution</td>
			<td>ThermoFisher Scientific</td>
			<td>AM7020</td>
			<td>Per biopsy: q.s</td>
		</tr>
		<tr>
			<td>Item: Sterile forceps 
			Sterile forceps</td>
			<td>Rocialle</td>
			<td>RML109-006</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: Sterile gauze swabs 
			Non woven swabs sterile 7.5 x 7.5 cm</td>
			<td>Prestige</td>
			<td>1860</td>
			<td>Per biopsy: 5</td>
		</tr>
		<tr>
			<td>Item: Sterile gloves 
			Prestige soft vinyl sterile powder free medical gloves</td>
			<td>Prestige</td>
			<td>S: P4301 
			M:P3302 
			L:P3301</td>
			<td>Per biopsy: pair</td>
		</tr>
		<tr>
			<td>Item: Sterile Microcentrifuge tubes 
			1.5 mL Sterile Microcentrifuge Tubes</td>
			<td>StarLab</td>
			<td>I1415-5510</td>
			<td>Per biopsy: q.s</td>
		</tr>
		<tr>
			<td>Item: Sterile sheet 
			Paper plain white 90 x 90 cm</td>
			<td>Rocialle</td>
			<td>RML 126-216</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>Item: Weighing boat 
			Diamond shape weigh boats</td>
			<td>Heathrow Scientific</td>
			<td>HS1427C</td>
			<td>Per biopsy: 1</td>
		</tr>
		<tr>
			<td>* denotes optional materials</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

human subcutaneous adipose tissue sampling using a mini-liposuction technique

对脂肪组织的研究有助于了解代谢和其他疾病。人体皮下脂肪组织是可及的。通过适当的培训和严格遵守无菌技术，研究人员可以在非临床环境中安全有效地获得皮下脂肪样本。在脐部外侧施用局部麻醉剂后，将连接到5或10mL注射器的14G针头通过皮肤插入皮下组织。在抽吸下，注射器以往复、切片运动移动以分离脂肪组织碎片。抽出柱塞足以确保脂肪组织碎片通过针头吸入注射器。单次活检可收集约200毫克组织。这种活检技术对参与者和研究人员来说都是非常安全的。活检后，参与者可以恢复大多数日常活动，尽管他们应该避免游泳和过度剧烈的活动48小时，以避免出血过多。参与者可以在一天内安全地进行2次活检，这意味着该技术可以应用于前后的急性干预研究。

所描述的脂肪组织活检程序是研究人员从人类志愿者那里获得皮下脂肪组织样本的一种有效且低风险的技术。我们使用上述程序对11名健康，体重正常的女性（年龄，27.4±3.3岁;体重指数（BMI），22.6±1.5 kg.m2）进行了39次皮下脂肪组织活检。所有参与者在禁食8-12小时后于07：00至10：00之间前往实验室。使用该脂肪组织活检程序的样品产量为192.0±97.1mg（范围= 32.8-393.6mg）（图4</...

Watch this Scientific Journal Video about Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique at JoVE.com

Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique

手稿和相关视频展示了一种经皮活检技术，可从脐周区域获取皮下脂肪组织样本。该方法是研究脂肪组织内一系列参数（例如，基因或蛋白质表达、酶活性、脂质含量）的低风险和有效方法。

使用微型吸脂技术进行人体皮下脂肪组织取样

Studies on adipose tissue are useful in understanding metabolic and other conditions. Human subcutaneous adipose tissue is accessible. With appropriate ...

该技术特别适用于任何实施研究前后设计或想要表征特定人群并且不需要过多身体后组织的研究。主要优点是该技术相对具有成本效益且几乎无痛。您可以在同一天对同一参与者进行多次活检。该技术可以帮助了解脂肪组织生理学，特别是对疾病和代谢功能的理解。首先，确保实验室有一个适当的私人房间，具有干净的可擦拭，无孔表面，以及一张干净舒适的床，参与者可以仰卧在上面。然后使用70%乙醇喷雾和干净的纸巾清洁活检程序所需的所有表面。接下来，将适当的锐器一次性垃圾箱和生物危害腰袋放在将进行活检的区域和进行活检的人附近。在参与者到达实验室之前，在新清洁的一般医疗推车上设置手术所需的设备。获得书面知情同意后，要求参与者填写书面问卷，以确保他们对此过程中使用的任何材料不过敏。在口头确认参与者的身份后，确保参与者了解要执行的程序和潜在的继发性副作用，包括瘀伤、疼痛和感染。除书面知情同意外，还需要参与者的口头同意。接下来，向参与者描述该过程，强调麻醉剂和活检的给药可能感觉如何。此外，请确保参与者对继续操作感到满意。再次，确保参与者对局部麻醉剂，某些金属和贝类没有过敏，并且参与者没有服用任何形式的抗凝药物。一旦参与者仰卧位，确定脐外侧约 5 至 10 厘米的活检部位。接下来，根据标准医疗指南用肥皂和温水洗手。将无菌床单放在干净的手推车或工作区域，注意只接触床单的外边缘。使用适当的无菌技术戴上无菌手术手套。然后让助手打开其余设备，使其掉落到准备好的无菌片上，而不会有任何接触或污染。确保助手在从无菌包装中取出物品时不会触摸物品。接下来，指示助手在一些无菌纱布上分配少量碘溶液。然后以螺旋运动从建议的活检部位向外移动对皮肤进行消毒。用新鲜的无菌纱布擦拭去除多余的液体（如果有）。与助手口头确认局部麻醉瓶的内容和有效期。然后指示助手将打开的小瓶倒置。并使用21号针头将5毫升局部麻醉剂吸入注射器中。将针头丢弃到尖锐的旋转中，并确保注射器没有气泡。接下来，将 26 号针头涂在注射器上并排出任何气泡。然后轻轻捏住腹部皮肤和脂肪组织，使其远离腹壁，并将针头水平插入皮下组织，角度相对于皮肤表面不超过10度。接下来，取出注射器的柱塞和额外的0.5毫升。如果注射器中出现血液，请以不同的角度抽出并重新插入针头，然后抬起直径为两到四毫米的气泡以麻醉插入区域。将针头推进皮下组织，并以扇形模式施用一毫升利多卡因。取出并处理 26 号针。然后将21号针头涂在注射器上，并以扇形模式施用剩余的四毫升利多卡因。使用无菌手术刀五分钟后，轻轻推动活检区域，以确保局部麻醉已生效并确定麻醉区域的边界。然后再等一两分钟，然后重新评估。一旦确信麻醉正在起作用，轻轻捏住皮肤和脂肪组织，并在皮肤上进行一到两毫米的小穿刺。接下来，将 14 号针头涂在 5 或 10 毫升注射器上。然后在轻轻捏住皮肤和脂肪组织的同时，轻轻地将针头通过穿刺插入麻醉区域的脂肪组织中，相对于皮肤表面的角度不超过10度。通过将柱塞抽出到大约 2.5 毫升标记来施加吸力。然后通过快速、前后移动针头来切片脂肪组织碎片来收集活检。30秒后，将针头和注射器扭转90度并重复此过程以分解脂肪组织的碎片，然后通过抽吸将其吸入注射器中。大约一分钟后，取下针头并将注射器内容物倒在覆盖在凹陷舟上的纱布上，确保针头的管腔朝下以避免潜在的血液飞溅。按照所示重复活检收集过程最多三次，并在每次重复该过程之前检查参与者是否满意。在收集其他样品时，指示助手处理和准备先前收集的样品进行分析或储存。收集满意的样品后，在穿刺伤口上放置一到两层无菌纱布。然后放置一个冰袋并坚定地施加压力约 10 分钟以诱导体内平衡。一旦发生体内平衡，用无菌纱布擦去任何碘溶液或干血，在部位涂抹带有吸收垫的粘性伤口敷料。在检查参与者感觉良好后，提供有关活检现场善后护理的口头和书面说明，强调参与者可能会在接下来的几天内表现出一些瘀伤。将用过的锐器和受污染的材料清理到指定的锐器或医疗废物容器中，然后使用70%乙醇清洁活检过程中使用的所有表面，并将一次性和非一次性床上用品分别放入适当的临床袋中或处置或清洁。在这项研究中，对11名健康正常体重的女性进行了39次皮下脂肪组织活检。使用该程序，平均获得192毫克样品。选择离脐部太近的活检部位会导致不必要的广泛瘀伤。本文总结了受试者在脂肪组织活检后经历的并发症列表。确保始终捏紧皮肤，以避免针刺穿更深的肌肉层或穿过皮肤的另一侧。实践可以完美地确保在对实际参与者进行尝试之前，由训练有素的医生进行培训。当插入针头以分解脂肪组织时，请确保进行活检的人有足够的把握来分解样本。

Research

JoVE Journal

Medicine

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

肩胛间褐色脂肪组织的切伦科夫发光成像

Brown adipose tissue (BAT), widely known as a &#8220;good fat&#8221; plays pivotal roles for thermogenesis in mammals. This special tissue is closely ...

科研

医学

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&#246;m Technique

Human Skeletal Muscle Biopsy Procedures Using the Modified BergstrÃƒÆ’Ã‚Â¶m Technique

人骨骼肌活检手术使用修改伯格斯坦技术

The percutaneous biopsy technique enables researchers and clinicians to collect skeletal muscle tissue samples. The technique is safe and highly ...

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

封装产热前脂肪细胞移植到脂肪组织仓库

Cell encapsulation was developed to entrap viable cells within semi-permeable membranes. The engrafted encapsulated cells can exchange low molecular ...

Working with Human Tissues for Translational Cancer Research

与人体组织转化为癌症研究工作

Medical research for human benefit is greatly impeded by the necessity for human tissues and subjects. However, upon obtaining consent for human ...

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

使用渗透皮下血管紧张素Ⅱ输液泵诱导主动脉瘤小鼠

Osmotic pumps continuously deliver compounds at a constant rate into small animals.  This article introduces a standard protocol used to induce aortic ...

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

使用层压技术来执行人类巩膜共聚焦显微镜


The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and ...

A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method

经非透法皮下腹脂肪组织活检技术

Adipose tissue biopsies offer tissue samples that, upon analysis, may provide insightful overviews of mechanisms relating to metabolism and disease. To ...

Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy

应用 Videomicroscopy 评价人脂肪组织微血管功能

While obesity is closely linked to the development of metabolic and cardiovascular disease, little is known about mechanisms that govern these processes. ...

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

应用流式细胞仪对人体脂肪组织免疫细胞的表征

Infiltration of immune cells in the subcutaneous and visceral adipose tissue (AT) deposits leads to a low-grade inflammation contributing to the ...

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

18F-fdg Petct 对活性人棕色脂肪组织的全身和区域定量

In endothermic animals, brown adipose tissue (BAT) is activated to produce heat for defending body temperature in response to cold. BAT&#39;s ability to ...