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DOI :
10.3791/62650-v
March 31st, 2022
Chapters
0:04
Introduction
0:39
Enhancer Candidate Sequence Cloning into the AAV Vector Plasmid
3:09
Intracranially Inject rAAV-Packaged Plasmid into Neonatal Mice
5:32
Image and Analyze Brain Tissue Sections for Enhancer Activity
8:10
Results: AAV Deployment of Enhancer-Based Expression Constructs in Mouse Brain
9:21
Conclusion
이 프로토콜은 새로운 rAAV 기반 과도 인핸서-리포터 분석을 설명합니다. 이 분석은 마우스 뇌 에서 생체내에서 인핸서 구동 발현을 유도하는데 사용될 수 있다.
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