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DOI :
10.3791/62698-v
June 30th, 2021
Chapters
0:04
Introduction
1:00
Performing the Craniotomy and Exposing the Somatomotor Cortex
2:21
Injecting Virus into the Somatomotor Cortex
3:00
Creating a Spinal Window for Precise Spinal Cord Injections
5:31
Direct Injections into the Spinal Cord Targeting Axon Terminals
6:33
Results: Transduction of Neurons in the Motor Cortex
6:52
Conclusion
该协议展示了一种通过将顺行性化学遗传修饰剂注射到体细胞运动皮层和逆行可运输的Cre重组酶到颈脊髓中,将基因疗法应用于出生后5-10天新生大鼠细胞亚群的新方法。
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