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DOI :
10.3791/62702-v
•
10:22 min
June 29th, 2021
Chapters
0:04
Introduction
1:04
Complement Inactivation of Human Sera
2:05
Humanized-RBL Cell Culture
4:07
Passive Sensitization of Humanized-RBL Cells
4:57
Antigen-stimulated Degranulation and Mediator Release
6:19
β-hexosaminidase Activity Measurement
7:21
Results: Effect of Allergen on β-hexosaminidase Release
9:38
Conclusion
ここでは、メディエーター放出アッセイを提示し、ヒトIgE受容体にトランスフェクトしたラット好塩基性白血病細胞株を用いて、1型アレルギー反応において典型的に認められるエフェクター細胞の脱顆粒をシミュレートする。この方法は、高感度、再現性、およびカスタマイズ可能な方法でアレルゲンの生物学的活性を調査します。
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