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DOI :
10.3791/62741-v
•
7:29 min
August 13th, 2021
Chapters
0:04
Introduction
0:52
Preparation of Zebrafish
1:51
Fluorinated Ethylene Propylene (FEP) Tubes Preparation
2:54
2% Agarose Dish and Embedding Media Preparation
3:29
Sample Mounting
5:03
Sample Positioning
5:40
Image Acquisition and Processing
6:13
Results: Imaging of the Developing Heart
6:54
Conclusion
我们描述了用光片荧光显微镜 在体内 研究斑马鱼心脏的优化工具。具体而言,我们建议使用明亮的心脏转基因系,并提出新的温和嵌入和固定技术,以避免发育和心脏缺陷。还提供了适用于心脏成像的可能的数据采集和分析管道。
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